The company's Bibby Group recently acquired PCRmax, which improved the original Ilummina's Quantitative PCR instrument ECO 48 and upgraded to PRIME PRO 48. Looking at the market and demand for real-time PCR, you know that this product is quite competitive with its unparalleled temperature uniformity, data accuracy and rapid experimentation.
PCR market situation
The PCR market is divided into three areas: life science research, food and agriculture, and medical diagnosis. The life sciences research market is growing rapidly. Some surveys show that the PCR market has a global growth rate of 8%.
Among them: qPCR accounts for 64% of the total PCR market.
1. Mainly target DNA quantification and gene expression
2, for infectious diseases, prenatal diagnosis, screening of pathogens in food, qPCR kit market growth of transgenic detection
Thermal module system
Temperature uniformity is at the heart of the qPCR system. Temperature uniformity determines the quality of the experimental results. The uniform time determines the running time. The reproducibility of the same experiment for multiple plates and multiple instruments is also related to temperature uniformity. Most thermal cyclers, the entire thermal module has a temperature difference of ±0.5 °C even at steady state, which can easily lead to poor results; it may take 15-30 seconds to achieve uniform temperature, resulting in longer run times.
BIBBY's TECHNE brand PRIME PRO 48, thermal module with Novec's hollow gold-plated silver module, precision mold opening, brushless electrode design, XLL (extra long life) single peltier module, its temperature uniformity is ± 0.1 ° C; Excellent 5 times the thermal performance of other brands, can be said to be unparalleled.
The benefits of high temperature uniformity are:
1. Reduce the need for repeated samples: save samples and save operating costs (samples and reagents)
2, the accuracy of the results is credible: between the holes, between the plates, the results between the instruments are credible
3, a wide range of application capabilities: HRM, high confidence NGS library quantitative, methylation research
It can be said that accurate 48-well PCR has higher throughput than inaccurate 96-well PCR.
Optical system
In addition to the thermal module system, another core component of QPCR is the optical system. In other plate mode qPCR systems, the same excitation energy is used for all sample wells throughout the PCR process, so that the luminescence energy between the different wells is very different, resulting in strong signal holes causing crosstalk to adjacent holes.
The Prime Pro 48 uses a unique ALC technology. By controlling the excitation light time of the different wells, the different fluorescence intensities in the 48 wells can be homogenized in each cycle.
The details are as follows: 1) Before performing PCR, Prime Pro 48 will perform a pre-scan on all 48 wells to detect the initial LED exposure intensity of each well as a baseline for the fluorescence intensity of the whole plate. 2) After pre-scanning, Prime Pro 48 will set different LED exposure durations for each hole to reduce the fluorescence interference between the holes, keeping the entire plate with similar excitation light intensity and enhancing experimental accuracy. There are two 48 LEDs arrays (Blue/Green).
Characteristics
In summary, TECHNE's PRIME PRO 48 fluorescence quantification offers the following advantages, even when compared to 96-well instruments:
? Excellent five times thermal performance (temperature uniformity ± 0.1 ° C)
Industry-leading accurate data: Accurate 48-well PCR has higher throughput than inaccurate 96-well PCR
? Experimental fast (40 cycles standard 40 minutes, fast 20 minutes)
? High cost performance: 30% lower than traditional
Open platform, reagents, authorization to open
Therefore, PRIME PRO 48 has considerable market competitiveness. Even with only 48 holes, the PRIME PRO 48 is a very good choice from the user's point of view.
Guangzhou Special Instrument Technology Co., Ltd., as the general generation of BIBBY in the Greater China region, is responsible for the sales, marketing, consulting and services of this product.
About the language and the British Bibby / Germany ART / Germany CAT:
Guangzhou Special Instrument Technology Co., Ltd. specializes in general instruments such as laboratory sample preparation such as agitator/dispersive emulsifier, analytical instruments such as melting point/photometer, and life science instruments such as PCR. As the first generation of Bibby in South China, Guangdong, Guangxi, Sichuan, Chongqing, Yunnan, Hainan, Guizhou and Tibet are our services. The company is also the German ART, the first generation of German CAT in China.
British BIBBY was founded in the 1950s as one of the UK's largest manufacturers of laboratory scientific instruments and instruments, one of the world's most extensive range of laboratory instrument manufacturers, offering high quality and high quality products to the world. Known for operational performance. It has four sub-brands: Stuart, Techne, Jenway, Electrothermal.
Stuart: Specialized laboratory instruments such as sample pretreatment, including: colony counter, melting point meter, agitator, mixer, shaker, pure water distiller series;
Techne: Focus on molecular biology research equipment (gene amplification equipment and hybridization boxes), as well as temperature control product lines (including water baths and dry baths);
Jenway: An expert in analytical instruments such as UV/Spectrophotometers, Flame Photometers, and Colorimeter;
Electrothermal: As a new member of BIBBY with more than 70 years of history, the world's leading scientific instrument provider offers electric heating sets, parallel reaction equipment, Kjeldahl nitrogen equipment, and electronic Bunsen burner series. Its parallel reaction workstation is the global market leader.
Founded in the last century, Germany ART is the most professional dispersing emulsifier in Germany and the world. Its top-grade dispersing emulsified products range from laboratory instruments to pilot products to industrial equipment. There are many types of dispersing heads to meet customers' various needs. The application fields cover chemical, cosmetic, pharmaceutical, food, environmental protection and other fields.
Founded in the 1950s, CAT Germany is one of the experts in German sample preparation equipment. Its agitator, from hand-held, teaching, to scientific research, high-viscosity type, is the representative product line of CAT; nowadays it is from ordinary electronic motor to brushless motor, product miniaturization, leading the development of agitator trend.
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