Detection range: 96T30pg/ml-800pg/ml Use purpose: This kit is used to determine the content of prolactin (PRL) in rat serum, plasma and related liquid samples. Experimental principle This kit uses a double antibody sandwich method to determine the level of rat prolactin (PRL) in the specimen. The microplate was coated with purified rat prolactin (PRL) antibody to prepare a solid phase antibody, and prolactin (PRL) was sequentially added to the microcapsule of the coated monoclonal antibody, followed by HRP-labeled prolactin (PRL) antibody. The antibody-antigen-enzyme-labeled antibody complex was formed by binding, and after thorough washing, the substrate TMB was added for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The depth of the color is positively correlated with prolactin (PRL) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of rat prolactin (PRL) in the sample was calculated from a standard curve. Kit composition 1 30 times concentrated washing solution 20ml × 1 bottle 7 Stop solution 6ml × 1 bottle 2 Enzyme standard reagent 6ml × 1 bottle 8 standard product (1600pg / ml) 0.5ml × 1 bottle 3 enzyme label coating plate 12 holes × 8 9 standard dilutions 1.5ml × 1 bottle 4 sample dilution 6ml × 1 bottle 10 instructions 1 part 5 color reagent A liquid 6ml × 1 bottle 11 sealing film 2 sheets 6 coloring agent B liquid 6ml × 1 Bottle 12 sealed bag 1
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