Some suggestions on the treatment of fecal specimens
The detection of stool samples has important reference value for judging digestive system diseases. When collecting, it is required to use clean bamboo sticks to select feces containing abnormal pathological components such as mucus, pus and blood. Feces with no abnormal appearance must be collected from the surface, deep and multiple ends of the feces. When looking for parasites and counting eggs, collect feces for 24 hours. The dysentery amoeba trophozoite should be checked immediately after defecation, and the material should be taken from the place with pus blood and softness, and kept warm for inspection. When examining the eggs of Schistosoma japonicum, the mucus and pus blood should be taken. At least 30g of feces should be kept when the larvae are hatched, and they must be processed as soon as possible. Examination of pinworm eggs must be carried out with a transparent film swab from the folds around the anus at 12 pm or early in the morning before defecation and microscopic examination immediately. Occult blood test (chemical method), fasting meat and food containing animal blood and iron, vitamin C, etc. 3 days before the test. All stool samples should be checked within 1 hour after collection to prevent damage to digestive enzymes and pH.
Simply mix well with a certain amount of PBS, and then centrifuge to take the supernatant for detection. Generally, 5000 revolutions is enough. The centrifugation time is generally 30 minutes. If the centrifugation effect is not good, you can extend the time. These should be operated according to specific circumstances. There is no fixed mode. Shanghai Yanjin Biotechnology Co., Ltd. specializes in selling ELISA kits of various brand price levels. Serving universities and immunology research units. Quality assurance and perfect after-sales service. And can be free to detect, better service for you.
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