Detection range: 96T3 pmol/L -80 pmol/L Purpose: This kit is used to determine the content of estradiol (E2) in human serum, plasma and related liquid samples. Experimental principle The kit uses a double antibody sandwich method to determine the level of human estradiol (E2) in the specimen. The microporous plate was coated with purified human estradiol (E2) antibody to prepare a solid phase antibody, and estradiol (E2) was sequentially added to the microcapsules of the coated mAb, followed by HRP-labeled estradiol (E2). The antibody binds to form an antibody-antigen-enzyme-labeled antibody complex, which is thoroughly washed and then added to the substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The color depth is positively correlated with estradiol (E2) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of human estradiol (E2) in the sample was calculated from a standard curve. Kit composition 1 30 times concentrated washing solution 20ml × 1 bottle 7 Stop solution 6ml × 1 bottle 2 Enzyme standard reagent 6ml × 1 bottle 8 standard product (160 pmol / L) 0.5ml × 1 bottle 3 enzyme label coating plate 12 holes ×8 9 standard dilutions 1.5ml × 1 bottle 4 sample dilution 6ml × 1 bottle 10 instructions 1 part 5 color reagent A liquid 6ml × 1 bottle 11 sealing film 2 sheets 6 coloring agent B liquid 6ml × 1 / bottle 12 sealed bag 1 specimen requirement 1. The specimens should be extracted as soon as possible after collection, and the extraction should be carried out according to the relevant literature. The experiment should be carried out as soon as possible after extraction. If the test cannot be performed immediately, the specimen can be stored at -20 °C, but repeated freezing and thawing should be avoided. Samples containing NaN3 could not be detected because NaN3 inhibited horseradish peroxidase (HRP) activity. Procedure 1. Dilution of the standard: This kit provides one original standard, which can be diluted in a small tube according to the following chart. 80 pmol/L No. 5 standard 150 μl of the original standard was added 150 μl of the standard dilution 40 pmol / L No. 4 standard 150 μl of the No. 5 standard was added 150 μl of the standard dilution 20 pmol / L No. 3 standard 150 μl No. 4 standard is added 150 μl standard dilution 10 pmol/L No. 2 standard 150 μl No. 3 standard Add 150 μl standard dilution 5 pmol/L No. 1 standard 150 μl No. 2 standard Add 150 μl standard dilution 2. Adding samples: Set blank holes separately (the blank control wells are not added with the sample and the enzyme standard reagent, the other steps are the same), the standard holes, and the sample holes to be tested. Accurately load 50 μl of the standard on the enzyme-labeled plate, add 40 μl of the sample dilution to the well to be tested, and then add 10 μl of the sample to be tested (the final dilution of the sample is 5 times). Add the sample to the bottom of the well of the microplate, try not to touch the wall of the well, and shake gently to mix. 3. Incubation: The plate was sealed with a sealing film and incubated at 37 ° C for 30 minutes. 4. Solution: Dilute 30 times concentrated washing solution with distilled water 30 times and reserve. 5. Wash: carefully remove the sealing film, discard the liquid, dry it, fill each well with washing liquid, let stand for 30 seconds and discard it. , repeat this 5 times, pat dry. 6. Add enzyme: Add 50 μl of enzyme labeling reagent to each well, except for blank wells. 7. Incubation: operation is the same as 3. 8. Washing: operation is the same as 5. 9. Color development: add 50 μl of color developer A, add 50 μl of color developer B, gently shake and mix, and avoid light at 37 °C. 15 minutes. 10. Termination: 50 μl of stop solution was added to each well to terminate the reaction (when the blue color turned yellow). 11. Measurement: The absorbance (OD value) of each well was measured sequentially with a blank air conditioner of zero and a wavelength of 450 nm. The measurement should be carried out within 15 minutes after the addition of the stop solution.
1.4 Ring Stretch Wrapping Machine: To wrap coil products individually using stretch film, knited mesh and anti-rust packing paper as packaging material. The advantage is fast packing speed and convenient operation raising the work efficiency greatly and lowering the labor intensity for the workers. This machine completely replaces the manual wrapping. It is easy to ship and store, because of excellent packaging results, strong tension force, repelling moisture, preventing dust and protecting the surface of the products.
H Series Ring Stretch Wrapping Machine
Automatic Stretch Wrapper,Ring Wrapper,Automatic Cornering Stretch Wrapper,Ring Stretch Wrapping Machine
Shandong Sinolion Machinery Corp. Ltd , https://www.packingline.nl